Specific biomembrane adhesion --Indirect lateral interactions between bound receptor molecules |
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| Authors: | CW Maier A Behrisch A Kloboucek DA Simson R Merkel |
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| Institution: | Lehrstuhl für Biophysik, E22, Technische Universit?t München, 85748 Garching, Germany, DE
Research Center Julich, Institute for Thin Films and Interfaces 4, 52425 Julich, Germany, DE
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| Abstract: | We studied biomembrane adhesion using the micropipet aspiration technique. Adhesion was caused by contact site A, a laterally
mobile and highly specific cell adhesion molecule from Dictyostelium discoideum, reconstituted in lipid vesicles of DOPC (L-α-dioleoylphosphatidylcholine) with an addition of 5 mol % DOPE-PEG2000 (1,2-diacyl-sn-glycero-3-phosphatidylethanolamine-N-poly(ethyleneglycol) 2000]). The “fuzzy” membrane mimics the cellular
plasma membrane including the glycocalyx. We found adhesion and subsequent receptor migration into the contact zone. Using
membrane tension jumps to probe the equation of state of the two-dimensional “gas” of bound receptor pairs within the contact
zone, we found strong, attractive lateral interactions.
Received 16 February 2001 |
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| Keywords: | PACS 87 15 Kg Molecular interactions membrane-protein interactions – 87 16 Dg Membranes bilayers and vesicles |
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