Active membranes studied by X-ray scattering |
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Authors: | A Giahi M El Alaoui Faris P Bassereau T Salditt |
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Institution: | 1.Institut für R?ntgenphysik,G?ttingen,Germany;2.Laboratoire Physico Chimie,Unité Mixte de Recherche 168 Centre National de la Recherche Scientifique/Institut Curie,Paris Cedex 05,France |
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Abstract: | In view of recent theories of “active” membranes, we have studied multilamellar phospholipid membrane stacks with reconstituted
transmembrane protein bacteriorhodopsin (BR) under different illumination conditions by X-ray scattering. The light-active
protein is considered as an active constituent which drives the system out of equilibrium and is predicted to change the collective
fluctuation properties of the membranes. Using X-ray reflectivity, X-ray non-specular (diffuse) scattering, and grazing incidence
scattering, we find no detectable change in the scattering curves when changing the illumination condition. In particular
the intermembrane spacing d remains constant, after eliminating hydration-related artifacts by design of a suitable sample environment. The absence of
any observable non-equilibrium effects in the experimental window is discussed in view of the relevant parameters and recent
theories. |
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Keywords: | 87 64 Bx Electron neutron and X-ray diffraction and scattering 87 15 Kg Molecular interactions membrane-protein interactions 87 15 Ya Fluctuations |
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