基于脉冲时延估计的荧光寿命表征方法

荧光检测和分析技术中，荧光寿命的精确测量具有重要意义。针对纳秒级荧光寿命在流式细胞分析系统中无法直接测量的问题，提出一种基于互相关算法的脉冲时延估计荧光寿命表征方法。该方法将改进的线性调频Z变换算法与相关峰内插算法相结合，并与标准FFT算法仿真做对比，通过对哺乳动物细胞的荧光寿命进行测量，将所得数据进行处理，验证线性调频Z变换(MCZT)和相关峰内插(FICP)算法性能。实验结果表明，使用该算法降低了FFT计算带来的栅栏效应，可以提高互相关函数的分辨能力，测量荧光寿命的相对误差提高了4.344 3%。

Fluorescence lifetime representation method based on impulse delay estimation

The precise measurement of fluorescence lifetime is of great significance in the fluorescence detection and analysis techniques. Aiming at the problem that the nanosecond fluorescence lifetime cannot be measured directly in the flow cytometry system, a fluorescence lifetime characterization method based on the cross correlation algorithm was proposed, which combined the chirp Z transform (modified chirp Z-transform, MCZT) algorithm with the correlation peak interpolation (fine interpolation of correlation peak, FICP) algorithm.The comparison with the standard fast Fourier transformation (FFT) algorithm was conducted, the fluorescence lifetime of mammalian cells was measured, the obtained data was processed, and the performances of MCZT and FICP algorithms were verified. The experimental results show that the algorithm reduces the fence effect caused by FFT calculation effectively, the resolution ability of the cross-correlation function is improved, and the relative error in measuring fluorescence lifetimes was increased by 4.344 3%.