Visualization of collagen regeneration in mouse dorsal skin using second harmonic generation microscopy |
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Authors: | T Luo J X Chen S M Zhuo K C Lu X S Jiang and Q G Liu |
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Institution: | (1) Institute of Laser and Optoelectronics Technology, Fujian Provincial key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, China |
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Abstract: | The purpose of this study is to highlight a clearer understanding of the process of collagen regeneration during wound healing.
By means of second harmonic generation (SHG) microscopy, the changes of collagen arrangement at the wound margin were analyzed
at 0, 3, 5, 7, 11 and 13 days post injury. The degree of collagen disorders associated with the healing process was quantitatively
obtained using the aspect ratio of polar plot image of collagen azimuthal angles and the healing status of collagen could
be estimated by arithmetical mean deviation (Ra) of the collagen SHG images. Our results suggest that SHG microscopy has potential advances in the collagen studies during
wound healing and the arrangement of collagen fibers gradually transformed from disorder to order so as to contract the wound.
It is capable of promoting clinical application of the noninvasive imaging tool and the analysis methods of collagen disorder
as an effective scar management for prevention and treatment about aberrant healing.
Original Text ? Astro, Ltd., 2009.
The article is published in the original. |
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