Study of the denaturation of human serum albumin by sodium dodecyl sulfate using the intrinsic fluorescence of albumin |
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Authors: | I M Vlasova and A M Saletsky |
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Institution: | 1.M. V. Lomonosov Moscow State University,Moscow,Russia |
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Abstract: | An analysis of the intrinsic tryptophan fluorescence of human serum albumin (HSA) confirms that the denaturation of HSA by
sodium dodecyl sulfate takes place in two stages for different pH levels: the first is the disintegration of globules and
the second is the complete unfolding of the amino acid chain of HSA. At pH levels below the isoelectric point (pI 4.7) of
HSA, denaturation proceeds through both stages, but when the pH is above pI, denaturation ceases in the first stage. |
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