Simulation of a stream of photon counts in single-molecule fluorescence fluctuation spectroscopy

We present a simulation model for a stream of photon counts that can be detected in fluorescence fluctuation spectroscopy systems from diffusing molecules undergoing spontaneous transitions between radiative and nonradiative states. Using our model, we can obtain various characteristics of a random stream of photon counts which allows us to test fluorescence fluctuation spectroscopy methods and to predict the results of full-scale experiments.