| Interaction Between Cytochrome c and the Hapten 2,4-Dinitro-fluorobenzene by Electrospray Ionization Mass Spectrometry |
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| 作者姓名： | 吴波 储艳秋 戴兆云 丁传凡 |
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| 作者单位： | 复旦大学化学系物理化学研究所，上海200433;复旦大学化学系物理化学研究所，上海200433;华东医院传染科，上海200040;复旦大学化学系物理化学研究所，上海200433 |
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| 摘 要： | 采用电喷雾质谱、H/D交换和紫外光谱测定了半抗原2,4-二硝基氟苯和模型蛋白细胞色素c的相互作用. 电喷雾质谱结果表明，随着2,4-二硝基氟苯体积比百分含量的增加，细胞色素c的构象可以由天然的折叠构象转变为部分非折叠构象，最后转变为完全非折叠构象.细胞色素c在平衡态的H/D交换结合电喷雾质谱实验结果进一步确认了上述构象变化. 紫外光谱结果表明半抗原的加入对辅基血红素上的亚铁离子和细胞色素c上His18和Met80的强场配位作用没有明显的影响.
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| 关 键 词： | 相互作用，细胞色素c，2 4-二硝基氟苯，电喷雾质谱 |
| 收稿时间： | 2007/12/12 0:00:00 |
| 修稿时间： | 2008/1/30 0:00:00 |
Interaction Between Cytochrome c and the Hapten 2,4-Dinitro-fluorobenzene by Electrospray Ionization Mass Spectrometry |
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| Institution: | Physical Chemistry Institute, Department of Chemistry, Fudan University, Shanghai 200433, China;Physical Chemistry Institute, Department of Chemistry, Fudan University, Shanghai 200433, China;Infectious Disease Department, Huadong Hospital, Shanghai 200040, China;Physical Chemistry Institute, Department of Chemistry, Fudan University, Shanghai 200433, China |
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| Abstract: | Allergic contact dermatitis is a delayed hypersensitivity reaction, which results from skin exposure to low molecular weight chemicals such as haptens. To clarify the pathogenic mechanism, electrospray ionization mass spectrometry (ESI-MS) and hydrogen/deuterium (H/D) exchange, as well as UV spectroscopy, were applied to determine the interaction between the model protein cytochrome c (cyt c) and the hapten 2,4-dinitro-fluorobenzene (DNFB). The ESI-MS results demonstrate that the conformation of cyt c can change from native folded state into partially unfolded state with the increase of DNFB. The equilibrium state H/D exchange followed by ESI-MS further confirms the above results. UV spectroscopy indicates that the strongfield coordination between iron of heme (prosthetic group) and His18 or Met80 of cyt c is not obviously affected by the hapten. |
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| Keywords: | Interaction Cytochrome c 2 4-dinitro-fluorobenzene ESI-MS |
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