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壳聚糖辐射裂解制备基因载体及其体外转染性能研究
引用本文:林福星,曾琨,杨文秀,汪谟贞,荣洁琳,谢娟,赵宇,葛学武.壳聚糖辐射裂解制备基因载体及其体外转染性能研究[J].化学物理学报,2017,30(2):231-238.
作者姓名:林福星  曾琨  杨文秀  汪谟贞  荣洁琳  谢娟  赵宇  葛学武
作者单位:中国科学院软物质化学重点实验室, 中国科学技术大学高分子科学与工程系, 合肥 230026,中国科学院软物质化学重点实验室, 中国科学技术大学高分子科学与工程系, 合肥 230026,中国科学院软物质化学重点实验室, 中国科学技术大学高分子科学与工程系, 合肥 230026,中国科学院软物质化学重点实验室, 中国科学技术大学高分子科学与工程系, 合肥 230026,安徽医科大学第一附属医院整形外科, 合肥 230031,安徽医科大学第一附属医院整形外科, 合肥 230031,安徽医科大学第一附属医院整形外科, 合肥 230031,中国科学院软物质化学重点实验室, 中国科学技术大学高分子科学与工程系, 合肥 230026
基金项目:This work was supported by the National Natural Science Foundation of China (No.81171829, No.51473152, and No.51573175) and the Fundamental Research Funds for the Central Universities (WK2060200012, WK3450000001). We also thank Prof. Li-hua Yang and Prof. Ye-zi You at the University of Science and Technology of China (USTC) for their kind help in providing experimental reagents and instruments
摘    要:Chitosan (CS) is expected to be an ideal gene carrier for its high biosafety. In this work, CS with low molecular weight were prepared through the γ-ray radiation on the acetic acid solution of CS. The CS chains were scissioned under the γ-ray radiation, and the molecular weight (MW) of CS decreased with the absorbed dose. When the absorbed dose was above 30 kGy, the molecular weight of CS decreased about an order of magnitude. The γ-ray-radiation-scissioned CS can e ectively bind with plasmid (pEGFP) through complex coacervation method, forming pEGFP/ γ-ray-radiation-scissioned CS complex particles with a size of 200-300 nm. The complex particles have good stability and little cytotoxicity. The in uitro gene transfection efficiencies of the pEGFP/ γ-ray-radiation-scissioned CS complex particles were investigated by fluorescence microscope and flow cytometry. The results showed that the gene vectors using γ-ray-radiation-scissioned CS as the carrier will possess better gene transfection efficiency than those using natural high-MW CS as the carrier. The higher the absorbed dose, the smaller the MW of CS and the better transfection efficiency of the corresponding gene vector. This work provides a green and simple method on the preparation of CS-based gene vectors with high efficiency and biosafety.

收稿时间:2016/9/23 0:00:00
修稿时间:2016/10/17 0:00:00
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