| 具有介孔结构的MnSiO3@Fe3O4@C纳米粒子的制备以及作为pH响应的T1-T2*双模MRI造影剂的研究应用 |
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| 引用本文： | 段北晨,徐鹏平,郭 振,陈乾旺.具有介孔结构的MnSiO3@Fe3O4@C纳米粒子的制备以及作为pH响应的T1-T2*双模MRI造影剂的研究应用[J].化学物理学报,2019,32(3):391-398. |
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| 作者姓名： | 段北晨 徐鹏平 郭 振 陈乾旺 |
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| 作者单位： | 中国科学技术大学材料科学与工程系，中国科学技术大学功能纳米实验室，合肥 230026,中国科学技术大学材料科学与工程系，中国科学技术大学功能纳米实验室，合肥 230026,中国科学技术大学生命科学学院，安徽省细胞动力学和化学生物学重点实验室，合肥 230027,中国科学技术大学材料科学与工程系，中国科学技术大学功能纳米实验室，合肥 230026 |
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| 摘 要： | 本文通过一个简单的、温和的方案制备了平均尺寸为120 nm，介孔结构的纳米粒子MnSiO3@Fe3O4@C. 粒子的细胞毒性微小，可以用作T1-T2*双模MRI造影剂. 酸性条件下MnSiO3@Fe3O4@C释放出大量的Mn2+缩短T1弛豫时间，提高成像分辨率. 超顺磁性的Fe3O4可以增强T2对比成像，检测病变组织. 类似于肿瘤微环境/细胞器的酸性PBS(pH=5.0)中Mn2+的释放率达到31.66%，约为中性条件(pH=7.4)下的7倍. 释放的Mn2+通过内吞作用被细胞摄取，经肾脏排出，细胞毒性实验表明，MnSiO3@Fe3O4@C具有低的细胞毒性，即使高浓度的200 ppm MnSiO3@Fe3O4@C对HeLa细胞的毒性也相对较小. 对荷瘤小鼠静脉注射定量MnSiO3@Fe3O4@C后，可以观察到一个快速增强的对比成像，给药24 h后，T1MRI信号显著增强，达到132%，而T2信号则明显降低至53.8%，活体MR成像证明了MnSiO3@Fe3O4@C可以同时作为阳性和阴性造影剂. 此外，得益于介孔MnSiO3优秀的酸敏感性，MnSiO3@Fe3O4@C可以作为一种潜在的药物载体，实现肿瘤的诊疗一体化.
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| 关 键 词： | 造影剂，双模，MR成像，MnSiO3@Fe3O4@C |
| 收稿时间： | 2018/5/14 0:00:00 |
Mesoporous MnSiO3@Fe3O4@C Nanoparticle as pH-responsive T1-T2* Dual-modal Magnetic Resonance Imaging Contrast Agent for Tumor Diagnosis |
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| Bei-chen Duan,Peng-ping Xu,Zhen Guo and Qian-wang Chen.Mesoporous MnSiO3@Fe3O4@C Nanoparticle as pH-responsive T1-T2* Dual-modal Magnetic Resonance Imaging Contrast Agent for Tumor Diagnosis[J].Chinese Journal of Chemical Physics,2019,32(3):391-398. |
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| Authors: | Bei-chen Duan Peng-ping Xu Zhen Guo and Qian-wang Chen |
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| Institution: | Hefei National Laboratory for Physical Sciences at the Microscale, Collaborative Innovation Center of Suzhou Nano Science and Technology, Department of Materials Science & Engineering, CAS High Magnetic Field Laboratory, University of Science and Technology of China, Hefei 230026, China,Hefei National Laboratory for Physical Sciences at the Microscale, Collaborative Innovation Center
of Suzhou Nano Science and Technology, Department of Materials Science & Engineering, CAS High
Magnetic Field Laboratory, University of Science and Technology of China, Hefei 230026, China,Anhui Key Laboratory for Cellular Dynamics and Chemical Biology and School of Life Sciences, University of Science and Technology of China, Hefei 230026, China and Hefei National Laboratory for Physical Sciences at the Microscale, Collaborative Innovation Center
of Suzhou Nano Science and Technology, Department of Materials Science & Engineering, CAS High
Magnetic Field Laboratory, University of Science and Technology of China, Hefei 230026, China |
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| Abstract: | Mesoporous structured MnSiO3@Fe3O4@C nanoparticles (NPs) were prepared via a facile and efficient strategy, with negligible cytotoxicity and minor side efforts. The asprepared MnSiO3@Fe3O4@C NPs hold great potential in serving as pH-responsive T1-T2* dual-modal magnetic resonance (MR) imaging contrast agents. The released Mn2+ shortened T1 relaxation time, meanwhile the superparamagnetic Fe3O4 enhanced T2 contrast imaging. The release rate of Mn ions reaches 31.66% under the condition of pH=5.0, which is similar to tumor microenvironment and organelles. Cytotoxicity assays show that MnSiO3@Fe3O4@C NPs have minor toxicity, even at high concentrations. After intravenous injection of MnSiO3@Fe3O4@C NPs, a rapid contrast enhancement in tumors was achieved with a significant enhancement of 132% after 24 h of the administration. Moreover, a significant decreasement of 53.8% was witnessed in T2 MR imaging signal. It demonstrated that MnSiO3@Fe3O4@C NPs can act as both positive and negative MR imaging contrast agents. Besides, owing to the pH-responsive degradation of mesoporous MnSiO3, MnSiO3@Fe3O4@C NPs can also be used as potential drug systems for cancer theranostics. |
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| Keywords: | Magnetic resonance imaging MnSiO3@Fe3O4@C Dual-modal Contrastagent |
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