| 表面增强拉曼光谱对于蛋白质二级结构的酰胺III谱带表征 |
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| 引用本文： | 田康振,曹长春,聂新明,王 稳,韩彩芹.表面增强拉曼光谱对于蛋白质二级结构的酰胺III谱带表征[J].化学物理学报,2019,32(5):603-610. |
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| 作者姓名： | 田康振 曹长春 聂新明 王 稳 韩彩芹 |
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| 作者单位： | 江苏师范大学物理与电子工程学院，江苏省先进激光材料与器件重点实验室，徐州 221116,中国人民解放军95979部队，新泰 271200,江苏师范大学物理与电子工程学院，江苏省先进激光材料与器件重点实验室，徐州 221116,江苏师范大学物理与电子工程学院，江苏省先进激光材料与器件重点实验室，徐州 221116,江苏师范大学物理与电子工程学院，江苏省先进激光材料与器件重点实验室，徐州 221116 |
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| 摘 要： | 本文利用斜角沉积法制备银纳米棒阵列基底用于蛋白质二级结构的检测,结合酰胺Ⅲ谱带光谱分析,建立了一种基于表面增强拉曼光谱(SERS)蛋白质二级结构的表征方法.用这种方法获得了两种典型蛋白质(溶菌酶和细胞色素C)的SERS信号.通过分析蛋白质骨架酰胺Ⅲ的光谱,研究了浓度对蛋白质折叠状态的影响.结果表明在一定范围内随着浓度的增加,溶菌酶的α-螺旋结构和β-折叠结构成分增加,而细胞色素C的二级结构基本保持不变.
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| 关 键 词： | 表面增强拉曼光谱，银纳米棒阵列基底，蛋白质二级结构 |
| 收稿时间： | 2018/11/24 0:00:00 |
Sensitive and Label-Free Detection of Protein Secondary Structure by Amide III Spectral Signals using Surface-Enhanced Raman Spectroscopy |
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| Kang-zhen Tian,Chang-chun Cao,Xin-ming Nie,Wen Wang and Cai-qin Han.Sensitive and Label-Free Detection of Protein Secondary Structure by Amide III Spectral Signals using Surface-Enhanced Raman Spectroscopy[J].Chinese Journal of Chemical Physics,2019,32(5):603-610. |
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| Authors: | Kang-zhen Tian Chang-chun Cao Xin-ming Nie Wen Wang and Cai-qin Han |
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| Institution: | Jiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou 221116, China,The 95979 Army of Chinese People''s Liberation Army, Taian 271200, China,Jiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou 221116, China,Jiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou 221116, China and Jiangsu Key Laboratory of Advanced Laser Materials and Devices, School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou 221116, China |
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| Abstract: | |
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| Keywords: | Surface-enhanced Raman spectroscopy Silver nanorod Protein secondary structures |
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