以蛋白质作为模板的Au-CuO双金属簇中性葡萄糖传感器

本文提出以牛血清白蛋白为载体合成葡萄糖敏感复合材料. 首先以牛血清白蛋白作为模板，合成了Au-CuO双金属纳米团簇(Au-CuO/BSA)，再与多壁碳纳米管(MWCNTs)复合，制备了新型葡萄糖传感材料(Au-CuO/BSA/MWCNTs). Au-CuO/BSA/MWCNTs能在中性条件下稳定且有效地检测葡萄糖. 利用扫描电子显微镜对Au-CuO/BSA/MWCNTs的形貌进行了表征. 使用循环伏安法测试了Au-CuO/BSA/MWCNTs的电化学性能. 葡萄糖检测实验表明，在中性环境中用Au-CuO/BSA/MWCNTs修饰的Au电极具有良好的葡萄糖检测能力，且具有较好的稳定性、准确性、重复性和选择性. 与现有的葡萄糖检测材料不同，使用牛血清白蛋白后，复合材料能在不用Nafion溶液的情况下牢固地固定在Au电极表面，减少使用Nafion固定敏感材料后存在的电流阻塞效应. 由于牛血清白蛋白的特殊效果，复合材料可以保存极长的时间；在室温下的封闭环境中，该复合材料能够保存3∽4个月后，仍具有超过80%的活性. 此外，使用牛血清白蛋白为模板制备葡萄糖敏感材料是可行性.

Protein Templated Au-CuO Bimetallic Nanoclusters toward Neutral Glucose Sensing

In this study, the application of bovine serum albumin (BSA) as a carrier to glucose-sensitive materials for the detection of glucose was proposed. Au-CuO bimetallic nanoclusters (Au-CuO/BSA) were prepared using BSA as a template, the new sensing material (Au-CuO/BSA/MWCNTs) was synthesized by mixing with multi-walled carbon nanotubes (MWCNT) and applied to non-enzymatic electrochemical sensors to detect glucose stably and effectively under neutral condition. The scanning electron microscopy was used to investigate the morphology of the synthesized nanocomposite. The electrochemical properties of the sensor were studied by cyclic voltammetry. Glucose detection experiments show that Au-CuO/BSA/MWCNTs/Au electrode has good glucose detection ability, stability, accuracy, repeatability, and high selectivity in neutral environment. Unlike existing glucose-sensitive materials, due to the use of BSA, the composite material is firmly fixed to the electrode surface without a Nafion solution, which reduces the current blocking effect on the modified electrode. The composite materials can be effectively preserved for extremely long periods, higher than 80% activity is maintained at room temperature in a closed environment for 3 to 4 months, due to the special effects of BSA. In addition, the feasibility of using BSA in glucose-sensitive materials is confirmed.