Fluorescence Spectroscopic Determination of the Critical Aggregation Concentration of the GnRH Antagonists Cetrorelix,Teverelix and Ozarelix |
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Authors: | Alexander Schneider Alexander Lang and Wolfgang Naumann |
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Institution: | 1.Allgemeine Biochemie,Technische Universit?t Dresden,Dresden,Germany |
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Abstract: | The fluorescence spectroscopic behavior of three 2-naphthylalanine containing Gonadotropin-releasing hormone (GnRH) antagonists
Cetrorelix, Teverelix and Ozarelix has been investigated concerning their aggregation process in comparison to the non-aggregating
peptide D-Phe6-GnRH. The aim of the present investigation consisted in developing a method to determine the critical aggregation concentration
for these decapeptides. This was achieved by monitoring the incorporation of the fluorescent probe 1-anilino-8-naphthalene
sulfonate (ANS) into aggregates and utilizing the modification of band shape and intensity of the intrinsic peptide fluorescence
emission depending on the analyzed peptide concentrations. Finally an approach for the explanation of the observed band characteristics
is presented analyzing the fluorescence of fragments Cetrorelix1–4 and Cetrorelix1–6. |
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