Unfolding Features of Bovine Testicular Hyaluronidase Studied by Fluorescence Spectroscopy and Fourier Transformed Infrared Spectroscopy |
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Authors: | Nina Pan Xiaoqiang Cai Kai Tang Guolin Zou |
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Institution: | (1) State Key Laboratory of Virology, Department of Biotechnology, College of Life Sciences, Wuhan University, Wuhan, 430072, China |
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Abstract: | Chemical unfolding of bovine testicular hyaluronidase (HAase) has been studied by fluorescence spectroscopy and Fourier transformed
infrared spectroscopy (FTIR). Thermodynamic parameters were determined for unfolding HAase from changes in the intrinsic fluorescence
emission intensity and the formations of several possible unfolding intermediates have been identified. This was further confirmed
by representation of fluorescence data in terms of ‘phase diagram’. The secondary structures of HAase have been assigned and
semiquantitatively estimated from the FTIR. The occurrence of conformational change during chemical unfolding as judged by
fluorescence and FTIR spectroscopy indicated that the unfolding of HAase may not follow the typical two-state model. |
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Keywords: | Hyaluronidase chemical unfolding fluorescence phase diagram FTIR |
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