Validated Spectroflurimetric Determination of Some H1 Receptor Antagonist Drugs in Pharmaceutical Preparations Through Charge Transfer Complexation |
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Authors: | Mohie K Sharaf El-Din Fawzia Ibrahim Manal I Eid Mary E K Wahba |
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Institution: | (1) Department of Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt; |
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Abstract: | A validated simple, rapid, and selective spectrofluorimetric method was developed for the determination of some antihistaminic
H1 receptor antagonist drugs namely ebastine (EBS), cetirizine dihydrochloride (CTZ), and fexofenadine hydrochloride (FXD).
The method is based on the reaction of the cited drugs with some Π acceptors namely p-chloranilic acid (CLA), tetracyanoethylene
(TCNE), and 2,3-dichloro-5,6-dicyano-p-benzoquinone (DDQ) to give highly fluorescent derivatives. The fluorescence intensity—concentration plots were rectilinear
over the concentration ranges of 0.2–3.0, 0.2–2.5 and 0.15–2.0 μg/ml for EBS with CLA, DDQ, and TCNE respectively; 0.5–7.0,
0.5–6.0, and 0.2–4.0 μg/ml for CTZ with the previously mentioned reagents, and 0.2–3.5, 0.5–6.0, and 0.2–3.5 μg/ml for FXD.
The factors affecting the formation of the reaction products were carefully studied and optimized. The method was applied
for the determination of the studied drugs in their dosage forms. The results obtained were in good agreement with those obtained
by the comparison methods. Reactions Stoichiometries of the complexes formed between the studied drugs and Π acceptors were
defined by the Job’s method of the continuous variation and found in 1:1 in all cases. |
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