On the Emission Intensity of Fluorescent Microspheres in Cardiac Tissue Images |
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Authors: | Eugene Gussakovsky and Yanmin Yang |
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Institution: | (1) Institute for Biodiagnostics, National Research Council Canada, 435 Ellice ave., Winnipeg, Manitoba, R3B 1Y6, Canada;(2) Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, R3E 3P5, Canada |
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Abstract: | Formulations for the total fluorescence intensity of fluorescent microspheres in slabs of cardiac tissue were determined experimentally
and theoretically. The tissue depth, at which the slab can be considered as a semi-infinite turbid medium, and critical layer
thickness, which accounts for the most emission intensity were evaluated to be 8–9 and 3–5 mm, respectively, for the cardiac
tissue. When fluorescent microspheres are linearly distributed across the slab depth, the mean absorption of them is proportional
to the sum of their normalized total emissions in the slab excited from both sides. The formulations may be used for the fluorescence
images analysis of cardiac and other biological tissues. |
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Keywords: | |
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