Fluorescence Properties of Dibenzofluorescein in Aqueous Solution |
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Authors: | Xian-Fu Zhang Qiang Liu Aiijun Son Qihang Zhang Fuqun Zhao Fushi Zhang |
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Institution: | (1) Department of Chemistry, Hebei Normal University of Science and Technology, Qinghuangdao, Hebei Province, 066004, People’s Republic of China;(2) Chemistry Department, Tsinghua University, 202 West, Gongwuguan, Beijing, 100084, People’s Republic of China |
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Abstract: | The deprotonation of dibenzofluorescein (DBFL), a long wavelength fluorescence probe, results in the simultaneous occurrence
of neutral form, monoanion and dianion under physiological conditions. The fluorescence properties of the former two cannot
be measured directly because they are always coexistent with some other species. By measuring the fluorescence under various
pHs we computed the fluorescence parameters for each species involved in the prototropic equilibria of DBFL, including each
species’ emission spectrum, excitation spectrum, emission and excitation maximum, fluorescence quantum yield and lifetime.
It was found that the monoanion is the most fluorescent chromospheres (Φ
f = 0.66, compared to Φ
f = 0.25 for dianion, 0.18 for cation and 0.0 for the neutral form). Together with the computed pK
as, we are able to suggest that the monoanion plays a major role under physiological conditions when DBFL is used as a fluorescence
probe, contrary to the assumption in literature. |
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Keywords: | Dibenzofluorescein Fluorescence Fluorescein Fluorescence probe |
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