A New Spectrofluorimetric Method for Determination of Trace Amounts 5-Hydroxytryptamine in Human Urine and Serum |
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Authors: | Qian Peng Chongqiu Jiang |
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Institution: | (1) College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, 250014, China |
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Abstract: | A new spectrofluorimetric method was developed for the determination of trace amount of 5-hydroxytryptamine (5-HT) in human
urine and serum samples. In the NaAc-HAc buffer solution of pH=5.80, 5-HT can react with formaldehyde-acetylacetone system
to form a new compound which sends yellow green fluorescence at 533nm and the enhanced fluorescence intensity is in proportion
to the concentration of 5-HT. Optimum conditions for the determination of 5-HT were also investigated. The dynamic range and
detection limit for the determination of 5-HT are 5.35×10−7∼1.07×10−4 mol/L and 2.08×10−7 mol/L, respectively. The developed method is simple, practical and can be successfully applied to determination of 5-HT in
human urine and serum samples. Moreover, the enhancement mechanisms of the fluorescence intensity in the 5-HT - formaldehyde-acetylacetone
system have been also discussed. |
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Keywords: | 5-hydroxytryptamine (5-HT) Acetylacetone Formaldehyde Spectrofluorimetry |
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