Spectroscopic Study of the Interaction of Styrylcyanine Dyes Sbo,Sil and Their Derivatives with Bovine Serum Albumin |
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Authors: | Email author" target="_blank">Eldar?N?KurtalievEmail author |
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Institution: | (1) Physics department, Samarkand State University, University blvd. 15, 140104 Samarkand, Uzbekistan |
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Abstract: | The spectral-luminescent characteristics of newly synthesized styrylcyanine dyes on the base of dyes Sbo ((E)-2-(4-(dimethylamino)styryl)-3-methylbenzod]oxazol-3-ium
iodide) and Sil ((E)-2-(4-(dimethylamino)styryl)-1,3,3-trimethyl-3H-indolium perchlorate) in aqueous solutions without and
in the presence of bovine serum albumin (BSA) were studied. It was established that the absorption spectra of dyes Tol-6,
Dbo-10 and Dil-10 with increasing amount of BSA appear new bands with λmax = 505 nm, λmax = 512 nm and λmax = 566 nm, respectively, whose intensity increases in proportion to the amount of albumin. The intensity of the glow of the
main band of fluorescence in the presence of BSA sharply increases. The binding constant (K) and the number of binding sites
(N) of studied dyes with BSA were determined. The dependence of binding constants with BSA on the dipole moment of dye molecules
was determined, which indicates that besides electrostatic forces of attraction between molecules styrylcyanine dyes with
BSA, hydrophobic interactions are essential. |
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Keywords: | |
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