首页 | 本学科首页   官方微博 | 高级检索  
     检索      


Parallel detection and quantitative analysis of specific binding of proteins by oblique-incidence reflectivity difference technique in label-free format
Authors:Jun Dai  Lin Li  LiPing He  KangCheng Ruan  HuiBin Lu  KuiJuan Jin  GuoZhen Yang
Institution:1. Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, 100190, China
3. Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China
2. Collaborative Innovation Center of Quantum Matter, Beijing, 100190, China
Abstract:In this work, we parallelly detected the specific binding between microarray targets including 12 different kinds of proteins and the probe solution containing five corresponding antibodies and quantitatively analyzed the interactions between CDH13 and solution phase anti-CDH13 at six different probe concentrations by oblique-incidence reflectivity difference (OIRD) method in label-free format. The detection sensitivity reached 10 ng/mL. The experimental results indicate that the OIRD method is a promising and competing technique not only in research work but also in clinic.
Keywords:oblique-incidence reflectivity difference(OIRD)  protein microarray  label-free detection  quantitative analysis
本文献已被 CNKI SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号