利用近红外光激发的光声血管造影成像

利用近红外光结合对应波长的光吸收增强对照剂来实现在体小鼠脑皮层血管的光声成像.近红外光在生物组织上有较深的穿透能力；而作为光吸收增强剂的外源染料吲哚菁绿注射到血液系统后则增加了血管对光的选择性吸收，从而增强了血管光声信号的强度.实验在不破坏脑头皮和头盖骨的前提下，通过静脉注射吲哚菁绿，获得小鼠脑皮层血管的光声造影成像.光声重建的血管网络与小鼠脑部解剖照片相当符合.实验结果证明近红外光结合光吸收增强剂的光声血管造影技术对于开展光声脑功能成像，光声分子成像提供了潜在的可行性. 关键词： 光声成像 光吸收增强剂 血管造影 光声信号

Photoacoustic angiography for mouse brain cortex using near-infrared light

Photoacoustic imaging is a novel noninvasive imaging technique of biological tissue that overcomes the overwhelming scattering of light in biological tissue by utilizing ultrasonic wave to translate the image signal. In this study, near-infrared light and indocyanine green as an optical contrast agent were used to achieve the cerebrovascular distribution by photoacoustic imaging. Near-infrared light penetrates deep into the brain tissues through the skin and skull, and the indocyanine green enhances the photoacoustic signals of the blood vessels. A photoacoustic angiography of vascular distribution in the mouse brain in vivo was acquired that matches the anatomical photograph well. The experimental result demonstrates the photoacoustic imaging with near-infrared light and optical contrast agent has the potential for functional and molecular biomedical imaging.