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H-22细胞声孔效应的应力阈值
引用本文:莫润阳,林书玉,王成会.H-22细胞声孔效应的应力阈值[J].物理学报,2011,60(11):114306-114306.
作者姓名:莫润阳  林书玉  王成会
作者单位:陕西师范大学物理学与信息技术学院,陕西省超声学重点实验室,西安 710062
基金项目:国家自然科学基金(批准号: 10974128)、陕西省自然科学基金(批准号:2010JM1002)和中央高校基本科研业务费专项资金 (批准号:GK201002009)资助的课题.
摘    要:理论和实验研究了超声空化场中的H-22型肝癌细胞产生可逆声孔效应的剪应力阈值.本文用1.37 MHz的聚焦声场,当超顺磁性纳米氧化铁在细胞悬液中的终浓度为410 μg/mL,换能器负载电功率为2 W,超声辐照60 s,细胞存活率90%以上时,有45.9±13.5%的细胞显示普鲁士蓝染阳性,暗示超声作用下,这些细胞表面曾出现可逆性微孔而使磁性微粒由此进入细胞内.利用无界自由空间微泡运动方程的球对称稳态解对实验条件下细胞膜表面的切变应力进行数值估算,结果表明,使H-22细胞产生可逆性声孔效应的微流剪应力阈值为697 Pa. 关键词: 声孔效应 磁性标记 微流 剪应力

关 键 词:声孔效应  磁性标记  微流  剪应力
收稿时间:2010-12-28

Threshold value of shear stress in H-22 cells generated sonoporation
Mo Run-Yang,Lin Shu-Yu and Wang Cheng-Hui.Threshold value of shear stress in H-22 cells generated sonoporation[J].Acta Physica Sinica,2011,60(11):114306-114306.
Authors:Mo Run-Yang  Lin Shu-Yu and Wang Cheng-Hui
Institution:Mo Run-Yang Lin Shu-Yu Wang Cheng-Hui (Shaanxi Key Laboratory of Ultrasonic,College of Physics and Information Technology,Shaanxi Normal University,Xi'an 710062,China)
Abstract:Based on a spherically symmetric bubble model, the threshold shear stress for H-22 cells which are irradiated by continuous focused ultrasound is estimated. On the condition that the final concentration of the superparamagnetic iron oxide (SPIO) in the cell suspension is 410 μg/mL, the focused ultrasound sonication is at a frequency of 1.37 MHz, the power from amplifer is 2 W, and H-22 cells are exposed to the ultrasound for 60 s, the labeling efficiency is about 45.9%±13.5%. Prussian blue staining confirms iron uptake and shows numerous blue-stained iron particles in the cytoplasm, while more than 90% labeled cells remained viable. The results show that the reparable sonoporation arises in plasma membrane and SPIO nanoparticles enter into the H-22 cells. Numerical calculations show that the shear stress acting on the cell is 697 Pa, which is the threshold shear stress for H-22 cell sonoporation.
Keywords:sonoporation  magnetic labeling  microstreaming  shear stress
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