基于近场光学的微球超分辨显微效应

在光学成像领域,由于受到衍射极限的限制,常规成像分辨率在200nm左右.科学的不断进步对更高分辨率有着迫切需求,如何突破这个极限来获得更高质量的高分辨率图像是热门研究领域.2011年提出了微球超显微技术:在原有的光学系统中,将直径几微米至几十微米的透明微球直接置于样品表面,就能够成倍提高传统光学显微镜的成像能力.微球超显微技术以其简单直接的特点,受到广泛关注.本文介绍了光学显微镜的研究背景以及国内外团队在微球超分辨显微技术方面的研究进展,包括通过在微球表面进行环刻同心环、中心遮挡和表面涂覆的方法来调节微球所产生的光子纳米喷射方面所开展的一系列研究,并进行了理论模拟和实验验证,进一步提升了微球的超分辨显微效应.最后,展望了今后微球超分辨显微技术的应用与发展方向.

Super-resolution microscopic effect of microsphere based on the near-field optics

In the field of optical imaging, the conventional imaging resolution is about 200 nm due to the diffraction limit. The higher resolution is urgently needed for further developing scientific research. Therefore, how to break through this limitation to acquire high quality and high resolution image has become a hot research topic. The microspheres with the size of tens of micrometers exhibit the ability to improve the imaging resolution of the conventional optical microscope by locating them directly on the sample surface. Due to its simplicity, the microsphere optical nanoscope technology is widely studied. This paper introduces the research background of the optical microscope and the research progress of microsphere optical nanoscope technology. At the same time, approaches to adjusting the photonic nanojet generated by the microspheres by fabricating concentric ringing, central mask, and surface coating of microspheres are reviewed. The possible reasons for this improved resolution are discussed. The applications and development of the microsphere ultra-microscopic technology in the future are discussed.