Spectral imaging of time-resolved anisotropy: theory and experiment |
| |
| Authors: | Yanzhou Zhou Qingruo Wang Jingsong He Lerong Lin Faculty of Automation Guangdong University of Technology Guangzhou China |
| |
| Institution: | Yanzhou Zhou,Qingruo Wang,Jingsong He,and Lerong Lin Faculty of Automation,Guangdong University of Technology,Guangzhou 510006,China |
| |
| Abstract: | Time-resolved fluorescence anisotropy on the nanosecond time scale is useful for the study of the rapid rotation of macromolecules. A system combining the capabilities of fluorescence spectral imaging with time-resolved fluorescence anisotropy and enabling the wide-field measurement of the spectroscopic parameters of fluorophores is discussed. The phasor approach is used to quantitatively analyze the time-resolved fluorescence anisotropy by transforming the polarized parallel and perpendicular components to... |
| |
| Keywords: | |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
