| Spatial Fourier-decomposition optical fluorescence tomography-theoretical investigation |
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| 作者姓名： | 刘诚 ;Dug Young Kim ;朱健强 |
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| 作者单位： | Physics Department of Xuzhou Normal University，Gwangju Institute of Science and Technology.Gwangju，South Korea，Shanghai Institute of Optics and Fine Mechanics.Chinese Academy of Sciences |
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| 基金项目： | This work was supported by Creative Research Ini- tiatives （aD Nano Optical hnaging System Research Group） of MOST/KOSEF. |
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| 摘 要： | A new three-dimensional （3D） optical fluorescent tomographic imaging scheme is proposed with structured illumination and spatial Fourier-domain decomposition methods for the first time. In this spatial Fourierdecomposition optical fluorescence tomography （SF-OFT）, the intensity of focused excitation light from an objective lens is modulated to be a cosine function along the optical axis of the system. For a given position in a two-dimensional （2D） raster scanning process, the spatial frequency of the cosine function along the optical axis sweeps in a proper range while a series of fluorescence intensity are detected accordingly. By making an inverse discrete cosine transformation of these recorded intensity profiles, the distribution of fluorescent markers along the optical axis of a focused laser beam is obtained. A 3D optical fluorescent tomography can be achieved with this proposed SF-OFT technique with a simple 2D raster scanning process.
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| 关 键 词： | 理论研究 傅立叶分解光学荧光层析术 激发光聚焦强度 余弦函数 |
Spatial Fourier-decomposition optical fluorescence tomography-theoretical investigation |
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| Institution: | [1]Physics Department of Xuzhou Normal University, Xuzhou 221009; [2]Gwang.ju Institute of Science and Technology, Gwangdu, South Korea; [3]Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800 |
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