| Research on optical biosensor with up-converting phosphor marker |
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| 作者姓名： | 赵永凯 周蕾 王静 黄立华 闫中强 黄惠杰 杨瑞馥 刘蕾 任冰强 王向朝 |
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| 作者单位： | Shanghai Institute of Optics and Fine Mechanics Chinese Academy of Sciences，Institute of Microbiology and Epidemiology Academy of Military Medical Sciences，Chinese Academy of Inspection and Quarantine，Shanghai Institute of Optics and Fine Mechanics Chinese Academy of Sciences，Institute of Microbiology and Epidemiology Academy of Military Medical Sciences，Shanghai Institute of Optics and Fine Mechanics Chinese Academy of Sciences，Institute of Microbiology and Epidemiology Academy of Military Medical Sciences，Shanghai Institute of Optics and Fine Mechanics Chinese Academy of Sciences，Shanghai Institute of Optics and Fine Mechanics Chinese Academy of Sciences，Shanghai Institute of Optics and Fine Mechanics Chinese Academy of Sciences Shanghai 201800 Graduate School of the Chinese Academy of Sciences，Beijing 100039，Beijing 100071，Beijing 100025，Shanghai 201800，Beijing 100071，Shanghai 201800，Beijing 100071，Shanghai 201800，Shanghai 201800，Shanghai 201800 |
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| 基金项目： | This work was supported by the National Natural Science Foundation of China under Grant No. 60378024. |
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| 摘 要： | An optical biosensor with up-converting phosphor (UCP) marker is developed for the sensitive rapid immunoassay to the specific biomolecule. UCP can emit visible light when excited by infrared light. Through detecting and analyzing the content of UCP particles on the test strip after immunoreaction, the concentration of target analyte in the sample can be obtained. The detection sensitivity to plague IgG is better than 5 ng/ml; to plague FI-Ab is better than 100 pg/ml; to plague Yersinia pestis cell is better than 3×104 CFU/ml. Good linear response characteristics and an excellent correlation (R2≥0.95) have been verified by quantitative detection results. In the practical application, detection results to 167 analytic samples have an excellent consistency with those obtained by reverse hemagglutination test. The up-converting phosphor technology (UPT) based biosensor has stable, reliable, and sensitive performances. It can meet the need of various bioassay applications.
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