Study of van Gieson's picrofuchsin staining on second-harmonic generation in type I collagen |
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| Authors: | Hanping Liu Zhengfei Zhuang Lingling Zhao Junle Qu Xiaoyuan Deng |
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| Institution: | [1]School for Information and Optoelectronic Science and Engineering, South China Normal University, Cuangzhou 510700; [2]Key Laboratory of Optoeleetronic Devices and Systems (Ministry of Education and Cuangdong Province), Institute of Optoelectronics, Shenzhen University, Shenzhen 518060 |
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| Abstract: | Second-harmonic generation (SHG) microscopy is a recently developed nonlinear optical imaging modality for imaging tissue structures with submicron resolution and is a potent tool for visualizing pathological effects of diseases. In this letter, we present our investigation on the influence of van Gieson's (VG) alcoholic picrofuchsin staining on SHG in type I collagen (from tendon-rich C57BL/6). Multi-channel imaging and spectra analysis show that the strong SHG signal produced in fresh collagen type I fiber has been greatly suppressed after VG staining, which indicates that staining may induce the structural or characteristic changes of SHG-dependent crystal formed by collagen constituents, such as glycine, proline, and hydroxyproline. |
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