| 哺乳动物尿液中钙镁离子浓度的1H NMR测定方法 |
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| 引用本文： | 江李苗,唐惠儒.哺乳动物尿液中钙镁离子浓度的1H NMR测定方法[J].波谱学杂志,2013,30(1):80-85. |
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| 作者姓名： | 江李苗 唐惠儒 |
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| 作者单位： | 1. 中国科学院生物磁共振分析重点实验室(筹), 波谱与原子分子物理国家重点实验室，武汉磁共振中心(中国科学院 武汉物理与数学研究所)， 湖北 武汉 430071；
2. 中国科学院大学，北京 100049 |
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| 基金项目： | 国家自然科学基金资助项目(20825520,20775086,20775087,21175149) |
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| 摘 要： | 哺乳动物体液中的钙镁离子浓度能够反映体内营养代谢的平衡与一些疾病的发生发展. 尿液中钙镁离子浓度的准确测量需要不受基质影响的便捷新方法. 该文使用过量乙二胺四乙酸(EDTA)为探针并通过1H NMR方法测定钙镁离子与EDTA络合物的含量，建立了一种快速原位的尿液钙镁离子浓度测量方法. 将此方法分别用于人、大鼠及小鼠的尿液测定，发现3类哺乳动物的尿液钙镁离子浓度存在显著的物种依赖性和较大的相同物种个体间差异. 上述为生物体液中钙镁离子浓度的快速定量测量提供了便捷方法，也提供了3类哺乳动物尿液钙镁离子浓度的基础数据.
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| 关 键 词： | 核磁共振(NMR) 钙离子 镁离子 尿液 |
| 收稿时间： | 2012-06-04 |
An NMR Method for Determining Concentrations of Calcium and Magnesium Ions in Mammalian Urines |
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| JIANG Li-miao,TANG Hui-ru.An NMR Method for Determining Concentrations of Calcium and Magnesium Ions in Mammalian Urines[J].Chinese Journal of Magnetic Resonance,2013,30(1):80-85. |
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| Authors: | JIANG Li-miao TANG Hui-ru |
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| Institution: | 1. Key Laboratory of Magnetic Resonance in Biological Systems, Wuhan Center for Magnetic Resonance, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics(Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences), Wuhan 430071, China; ;
2. University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | Concentration of the calcium and magnesium ions in mammalian biofluids carries important information on metabolic homeostasis for nutrients and pathophysiology of some diseases. Methods are in urgent need to achieve rapid quantitative measurements of these ions in urines with little matrix effects. In this work, we report a simple 1H NMR method for measuring urinary concentrations of these ions by using ethylenediaminetetraacetic acid (EDTA) as a probeand measuring EDTA complexes of calcium and magnesium ions. This method was applied to analyze human, rat and mouse urine samples. The results indicated that urinary concentrations of calcium and magnesium ions had a clear species dependence and inter-individual variation within the same species. The method offered a simple and rapid way for quantitative measurements of calcium and magnesium ions in biofluids in situ and provided some useful data for urinary calcium and magnesium ions for man, rats and mice. |
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| Keywords: | NMR calcium ion magnesium ion urine |
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