| 胸腺五肽的NMR研究 |
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| 引用本文： | 刘力军,仲伯华,缪振春. 胸腺五肽的NMR研究[J]. 波谱学杂志, 2005, 22(2): 201-207 |
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| 作者姓名： | 刘力军 仲伯华 缪振春 |
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| 作者单位： | 军事医学科学院 毒物药物研究所，北京 100850 |
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| 摘 要： | 应用改进的DPFGSE 1D-TOCSY 和1D-NOESY 核磁共振方法测定胸腺五肽的氨基酸残基，从重叠的1H谱中分离出各自旋系统内氢的亚谱信号；继而由DPFGSE 1D-NOE 技术测定肽链氨基酸残基的连接顺序. 结合二维相关谱对胸腺五肽的碳、氢信号做了全归属. 并进行了计算机分子模拟，结果与NOESY 实验相符.
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| 关 键 词： | 核磁共振 氨基酸序列 改进的1D-TOCSY 改进的1D-NOESY 胸腺五肽 |
| 文章编号： | 1000-4556(2005)02-0201-07 |
| 收稿时间： | 2004-11-01 |
| 修稿时间： | 2004-11-01 |
An NMR Study of Thymopoietin |
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| LIU Li-jun,ZHONG Bo-hua,MIAO Zhen-chun. An NMR Study of Thymopoietin[J]. Chinese Journal of Magnetic Resonance, 2005, 22(2): 201-207 |
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| Authors: | LIU Li-jun ZHONG Bo-hua MIAO Zhen-chun |
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| Affiliation: | Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China |
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| Abstract: | Severe spectral overlap occurs in the NMR spectrum of thymopoietin because the composing amino acid residuals have similar chemical shifts. In this paper, the structure of thymopoietin was determined by modified DPFGSE 1D-TOCSY and 1D-NOESY NMR techniques, which was then confirmed by computer simulation. The results demonstrate that DPFGSE 1D-TOCSY and 1D-NOE are efficient tools for structural elucidation of thymopoietin. |
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| Keywords: | NMR thymopoietin structure amino acid sequence 1D-TOCSY 1D-NOESY |
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