小白鼠肌肉组织的NMR质子自旋交换分析

本文在Zimmerman-Brittin两相质子交换核磁共振弛豫模型基础上,分析了NMR弛豫实验中检测信号与各相表现和本征弛豫多数的关系,编写了自动化处理实验数据的计算机程序,这一技术可用于复相系统中不同成分的NMR表现和本征弛豫特性研究中,本文中的样品是选用健康新鲜的小白鼠肌肉,没加任何处理,用h-h,s-h,s-s脉冲序列,反转恢复法(π-τ-π/2)在强场下(0.92T)做T₁、T₂测定实验,分析结果表明本征弛豫参数T₁=1050ms,T₂=4500μs的成分是由肌肉中的"自由水"引起的,其质子相对含量为69%;本征弛豫参数T₁=530ms,T₂=26μs的成分是由肌肉中的"束附水"引起的,其质子相对含量为9%,本征弛豫多数T₁=530ms,T₂=1250μs的成分是由肌肉中的各种大分子和有机物引起的,其质子相对含量为9%,本征弛豫参数T₁=470ms,T₂=1250μs的成分由样品中的脂肪引起的,其质子相对含量为13%,在肌肉组织中的质子与水中质子之间有强烈的交换作用,其交换率k=1000s^-1.在脂肪中的质子与其它成分之间没有交换作用。

NMR PROTON SPIN EXCHANGE ANALYSIS ON HEALTHY FRESH MOUSE MUSCLE TISSUES

Based on Zimmerman-Brittins model of spin exchange between two phases,the line-shape relaxation correlation technique, also called spin-grouping technique,was analysed.The programs processing experimental data are complied.This technique can be used in the study of NMR apparent and inherent relaxation characteristic of different components in heterogeneous system.In this paper the sample is the healthy fresh mouse muscle.T₁ experment(π-τ-π/2) was performed using h-h,s-h,s-s pulse sequence at high field(0.9T).The results indicate that the T₁=1050 ms,T₂=4500μs component is caused by "free water" in muscle, the proton relative content of which is 69%;the T₁=530 ms,T₂=26 μs component is caused by "bound water"in muscle,the proton relative content of which is 9%, the T₁=530 ms,T₂=1250μs component is caused by various large molecules and organic compounds in muscle,the proton relative content is 9%;the T₁=470 ms,T₂=1250 μs componentis eaused by the fat in the sample,the proton relative content is 13%. Between theproton in muscle tissues and the proton in water fast exchange is dominant but thereis no exchange between the proton in fat and other components.