磷烧伤的31P NMR研究

为了明确磷烧伤创面及吸收毒性物质的化学形式及分布.作者采用³¹P PNMR波谱,研究皮下注射黄磷、磷酸及磷烧伤后创面残留、肝脏及脑组织吸收的元素磷及其化合物含量.其结果皮下注射非致死与致死剂量的黄磷后,肝脏可测到元素磷的吸收,磷燃烧后创面磷绝大部分转化为磷酸及其结合物,极少部分以元素磷形式存在.致死面积的磷酸烫伤与磷烧伤后肝脏区域磷酸增加4~5倍,未检测到元素磷的波谱,各组脑组织均未检测到元素磷峰,而磷酸峰只有微小的增加.结论为磷烧烧后吸收的主要中毒物质与创面残留的主要化学物质一致,磷烧伤中毒的致死性主要是创面磷酸吸收所致.

THE 31P NMR INVESTIGATION OF PHOSPHORUS BURNS

Purpose:In order to investigate the chemical form and distribution of toxicants absorbed following phophorus burns(PB). Methods:Surface-coil \{\} 31 P NMR was used to study the contents of phosphorus (P\-4) and its metabolites in liver, brain and wounds after PB, Phosphate scald (PS), and subcutaneous injection of phosphorus (SIP). Results:Obvious sign of P\-4 in the liver but not brain was observed in both dead and survival animals following SIP. While after PB most P\-4 was changed into phosphate, phosphate increased significantly in the liver and mild in the brain with no sign of P\-4 after PB and PS. Conclusion: The chemical form of main toxicants absorbed was accompaned with those remained within wounds after PB. Phosphoric acid was fetal cause of phosphorus poisoning after PB.