N-异丁酰基-3'-O-(1-氟-1,1,3,3-四异丙基-1,3-二硅氧烷-3-基)-2'-苄氧羰基鸟苷的NMR研究

本文采用氢氟酸吡啶鎓在5′-位选择性断裂1，1，3，3-四异丙基二硅氧烷基保护基的方法得到了核苷衍生物N-异丁酰基-3′-O-（1-氟-1，1，3，3-四异丙基-1，3-二硅氧烷-3-基）-2′-苄氧羰基鸟苷（化合物1），并应用液相色谱串联质谱（LC-MS）、气相色谱-高分辨质谱（GC-HRMS）、液体1D和2D NMR谱（包括¹H NMR、¹³C NMR、¹⁹F NMR、DEPT、¹H-¹H COSY、¹H-¹³C HSQC和¹H-¹³C HMBC），对产物的¹H、¹³C和¹⁹F NMR信号进行了归属，确定了其结构．

An NMR Study of N-isobutyryl-3'-O-(1-fluoro-1,1,3,3-tetraisopropyl-1,3-disiloxane-3-yl)-2'-benzyloxycarbonyl-guanosine

The nucleoside derivative N-isobutyryl-3'-O-(1-fluoro-1,1,3,3-tetraisopropyl-1,3-disiloxane-3-yl)-2'-benzyloxy carbonyl-guanosine (compound 1) was obtained by selectively cleaving the 1,1,3,3-tetraisopropyldisiloxanyl protecting group at 5'-position with pyridinium hydrofluoride. Liquid chromatography-mass spectrometry (LC-MS), gas chromatography-high resolution mass spectrometry (GC-HRMS), liquid one-dimensional/two-dimensional nuclear magnetic resonance (NMR) techniques (i.e., ¹H NMR, ¹³C NMR, ¹⁹F NMR, DEPT, ¹H-¹H COSY, ¹H-¹³C HSQC and ¹H-¹³C HMBC) were used to assign the signals.