PAMAM树状大分子与5-氟尿嘧啶相互作用的NMR研究

本文利用多种核磁共振（NMR）技术研究了聚酰胺-胺树状大分子（PAMAM）与5-氟尿嘧啶（5-FU）在水溶液中的相互作用.¹H NMR化学位移滴定结果表明5-FU分子结合在PAMAM分子表面，¹³C NMR化学位移滴定分析结果表明5-FU分子通过氢键或疏水间相互作用进入到PAMAM的内部疏水空腔.¹H核和¹⁹F核自旋-晶格弛豫时间（T₁）的测定结果也说明了二者之间存在相互作用.NOESY实验进一步验证了5-FU分子进入到PAMAM分子的内部空腔.本研究为研究树状大分子和药物小分子的相互作用提供了系统的NMR分析方法.

Interactions Between 5-Fluorouracil and PAMAM Dendrimers Studies by NMR Spectroscopy

Interactions between 5-fluorouracil (5-FU) and PAMAM in D₂O solution were studied by NMR spectroscopy. The results of ¹H NMR chemical shift titration experiments demonstrated that 5-FU molecules bonded to the surface of PAMAM, and the results of ¹³C NMR chemical shift titrations experiments disclosed that hydrogen-bond interactions and/or hydrophobic interaction ins existed in the interior pockets of PAMAM and 5-FU. These results were further supported by ¹H and ¹⁹F relaxation time measurements. The NOESY experiment was used to confirm the presence of 5-FU encapsulation in the PAMAM-5-FU complexes. This study provides a methodology for studying the interactions between dendrimers and drug molecules.