涡虫代谢物组成的核磁共振分析

作为扁形动物门的代表动物，涡虫因其特殊的演化地位和极强的再生能力，迅速成为实验动物学、细胞遗传学、发育生物学和再生生物学等研究中的理想模式生物. 众多与涡虫相关的研究都取得了突破性进展，而其在核磁共振和代谢组领域的相关科研报道却极为匮乏. 该研究使用甲醇水提取方法获得了涡虫身体提取物样品，同时利用高分辨的1 GHz NMR谱仪对该样品采集了¹H NMR谱图和5种2D NMR谱图（¹H-¹H COSY，¹H-¹H TOCSY，¹H-¹H J-RES，¹H-¹³C HSQC和¹H-¹³C HMBC）. 通过归属得到涡虫身体提取物里所含有的53种代谢物质，包括氨基酸、糖类、脂类、有机羧酸、酮体、胺类、胆碱类以及核苷酸等. 这些物质覆盖了多种代谢途径，为涡虫代谢组研究提供了基本的NMR相关信息.

Metabolite Composition of Planarian Analyzed byNuclear Magnetic Resonance Spectroscopy

Planarian is a free-living worm belonging to the Phylum Platyhelminthes. Because of its special evolutionary status and remarkable regenerative abilities, planarian has emerged as an ideal model organism for the study of experimental zoology, cytogenetics, developmental biology and regenerative biology. Although significant advances have been made in recent studies of planarian, little information is available in metabolome of the worm. In this work, we prepared samples of planarian body extract with the aqueous methanol extraction method, and analyzed the samples with 1D ¹H NMR spectroscopy and 2D NMR spectroscopy (e.g., ¹H-¹H COSY, ¹H-¹H TOCSY, ¹H-¹H J-RES, ¹H-¹³C HSQC and ¹H-¹³C HMBC) on a high-resolution 1 GHz NMR spectrometer. Fifty three metabolites were identified from the planarian body extracts, including amino acids, saccharides, lipids, organic acids, ketone bodies, amines, choline and nucleotide metabolites. The results of this study will provide a basic dataset for planarian metabolome research.