| 形成素结合蛋白C-端FF结构域的1H和15N NMR谱峰归属和结构分析 |
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| 引用本文： | 吴昌琳,宋国强,胡红雨.形成素结合蛋白C-端FF结构域的1H和15N NMR谱峰归属和结构分析[J].波谱学杂志,2006,23(1):1-10. |
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| 作者姓名： | 吴昌琳 宋国强 胡红雨 |
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| 作者单位： | 华东师范大学,物理系,华东师范大学光谱学与波谱学教育部重点实验室,上海,200062;中国科学院,上海药物研究所,上海,201203;中国科学院,上海生物化学和细胞生物学研究所,上海,200031 |
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| 摘 要： | FF结构域广泛存在于与信号传导相关的蛋白质中，我们选择形成素结合蛋白11 (formin-binding protein 11, FBP11)中所含的一个FF结构域，通过在H2O和D2O中采集的COSY，TOCSY，NOESY等二维核磁共振谱，识别了FF-结构域的氨基酸残基的质子自旋系统，通过解析二维和三维NOESY谱中的dαN、dNN和dβN等NOE相关完成了序列专一性归属；二级结构分析表明FF-结构域包含三段α 螺旋.
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| 关 键 词： | 核磁共振 FF-结构域 序列专一归属 二级结构 |
| 文章编号： | 1000-4556（2006）01-0001-10 |
| 收稿时间： | 2005-06-07 |
| 修稿时间： | 2005-10-17 |
Assignment of 1H and 15N Chemical Shifts and Structure Elucidation of C-terminal FF-domain of the Formin-Binding Protein |
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| WU Chang-lin,SONG Guo-qiang,HU Hong-yu.Assignment of 1H and 15N Chemical Shifts and Structure Elucidation of C-terminal FF-domain of the Formin-Binding Protein[J].Chinese Journal of Magnetic Resonance,2006,23(1):1-10. |
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| Authors: | WU Chang-lin SONG Guo-qiang HU Hong-yu |
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| Institution: | Key Laboratory of Optical and Magnetic Reasonance Spectroscopy for Education Ministry, Physics Department of East China Normal University, Shanghai 200062, China |
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| Abstract: | FF-domains have been found widely in many signaling proteins. In this paper, the structure characteristics of FF-domain of the formin-binding protein 11 (FBP11) were studied by NMR spectroscopy. The spin systems for most of the amino acid residues in the FF-domain were identified by analyzing NMR spectra acquired in H2O and D2O. Sequence specific assignment of the spin systems was made using dαN, dNN and dβN NOEs correlation calculated from NOESY spectra. The secondary structure of the FF-domain was found to include three α helices. |
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| Keywords: | NMR spectroscopy FF-domain chemical shift assignment secondary structure formin-binding protein |
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