蛋白质支链动力学快运动的核磁共振研究

蛋白质的功能不仅取决于其结构，而且受到其构像及其变化的影响. 许多生物化学过程就是由于蛋白质结构的一些动力学变化而完成，如蛋白质-蛋白质，蛋白质-药物配体之间的相互作用. 因此分析蛋白质的动力学变化，就能够对其参与的生化过程进行分析. 作为动力学研究的有力工具之一，核磁共振能够分辨到原子范围内的从千秒到皮秒时间范围的运动过程，因此在动力学研究中有着不可替代的作用. 本文仅就核磁共振在蛋白质支链快运动方法（ps-ns）研究方面的进展进行总结，以期阐明核磁共振的在支链动力学研究中的发展现状.

NMR- Approaches for Probing Fast Time Scale Dynamics of Protein Side Chains

Dynamic aspects of proteins are increasingly recognized as being important for describing their biological functions such as protein-protein and protein-drug interactions. Consequently, considerable efforts have been made to characterize protein dynamic motions. Among these methods, nuclear magnetic resonance (NMR), in particular, is capable of providing valuable information on the nature of internal motions of proteins in solution. In this review, the NMR-based approaches for studying protein side chain dynamics are reviewed, with emphasis on those methods for probing fast time scale internal motions.