金纳米棒标记HepG2人肝癌细胞的荧光成像及其AFM探测

金纳米棒具有独特的光学性质,在生物医学领域有着广泛而重要的应用前景.本文制备了长径比为8∶1的金纳米棒,其在480 nm波长激发下,在560 nm和707 nm波长处有两个荧光发射峰.基于金纳米棒的荧光性质,将其标记于HepG2人肝癌细胞表面,利用激光扫描共聚焦显微镜对标记后的细胞进行荧光成像.在488 nm激发下,获...

Fluorescence Imaging of Hepatocellular Carcinoma Cells Labeled with Gold Nanorods and Detection by AFM

Due to the unique and tunable optical properties, gold nanorods have potential and important applications in the biomedical field. In this paper, gold nanorods with the aspect ratio 8 were prepared, an outstanding fluorescence feature of the gold nanorods excited at 480 nm was observed in terms of a strong fluorescence emission at 707 nm and a relatively weak fluorescence at 560 nm. Based on their fluorescence properties, HepG2 cells were labeled by this type of gold nanorods, and were studied by using laser scanning confocal microscope. Green and red fluorescence images of gold nanorods were collected under the excitation of 488 nm. Furthermore, high-resolution atomic force microscopy was used to obtain the morphology and mechanical properties changes of the HepGz cells.