不同波长激发光诱导奶牛血液荧光光谱特性

实验样品取自正常奶牛静脉血液并用枸橼酸钠抗凝。实验结果表明:激发光波长不同,激发的荧光光谱线的强度和峰值不同;实验中用220～270nm波长的光激发荧光时,在317,367nm荧光主峰强度出现竞争现象;比较多种激发光激发的荧光光谱的实验结果知:用220,230,240,290,350,480,500nm波长的激发光激发下的荧光强度较强,而用其他波长的激发光激发下的荧光强度较弱,且在317,365,367,388,348,463,467,607,638nm波长附近出现比较强的峰值;合适波长的激发光对奶牛血液会有比较强的生物学效应。

Research on Fluorescent Spectrum of Cow Blood Induced by Excitated Light with Different Wavelength

Aiming at the measurement of the cow bloods,the generation mechanism and characteristic of fluorescent spectra of cow blood were researched in this paper.The fluorescent excitation and spectrum measurement system is fluorescence spectrophotometer RF-5301(Shimadzu).The experimental sample was the normal blood that was drawn out from the cow vein.When added into some anti-coagulator,the blood samples' spectra were measured.These experimental cows were selected from the several farms in the province of Heilongjiang.They were all healthy cows but different in age.The experiment results show that the fluorescence of the cow bloods can be effectively induced by light wave.The intensity and peak value of the fluorescent spectra are different with the wavelength of the excitation light.In the experiment the wavelength value of fluorescence peaks are found around two places:one peak is near 317 nm and the other is near 367 nm when the wavelengths of the light ranges from 220 to 270 nm.At this time the strife phenomena occur.After we compared the experiment results about the fluorescent spectra with some different excitation light,we find that the fluorescent intensities which are excited by 220,230,240,290,350,480,500 nm light are greater.The fluorescent intensities which are excited by other light are lower and the great peak values appear at around 317,365,367,388,348,463,467,607,638 nm.It is shown that the excitation light would give obvious biologic effect on the cow bloods.This study is valuable for selecting of the best excitation light wave in the technology of fluorescent spectra of cow bloods induced by excitation light.It provides basic information for the early distinguishing and diagnosis of cow diseases in the application of the fluorescence spectrum technology.