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黄嘌呤氧化酶-乙醛-吖啶酯化学发光分析新体系
引用本文:张帆,庄惠生.黄嘌呤氧化酶-乙醛-吖啶酯化学发光分析新体系[J].发光学报,1996,17(3):245-250.
作者姓名:张帆  庄惠生
作者单位:福州大学化学系, 福州 350002
摘    要:乙醛在黄嘌呤氧化酶催化作用下,被空气氧化生成乙酸和过氧化氢,酶反应生成的过氧化氢氧化新吖啶酯试剂10-甲基-9-(对甲酰苯基)吖啶羧酸酯氟磺酸盐]产生化学发光,化学发光强度与己醛浓度在一定范围内成线性关系.由此建立了一种化学发光分析新体系,用于测定乙醇含量.线性范围2.0×10-7~1.2×10-3mol/L,测定5.0×10-7mol/L乙醛,相对标准偏差为6.8%,检测限为1.2×10-7mol/L,测定环境水样中乙醇含量,取得满意结果,回收率为95~106%.

关 键 词:黄嘌呤氧化酶  乙醛  化学发光分析  10-甲基9-(对甲酰苯基)吖啶羧酸酯氟磺酸盐
收稿时间:1995-08-23

A NEW CHEMILUMINESCENCE ANALYTICAL SYSTEM OF XANTHINE OXIDASE-ACETALDEHYDE-ACRIDINIUM
Zhang Fan,Zhuang Huisheng,Wang Qiong'e.A NEW CHEMILUMINESCENCE ANALYTICAL SYSTEM OF XANTHINE OXIDASE-ACETALDEHYDE-ACRIDINIUM[J].Chinese Journal of Luminescence,1996,17(3):245-250.
Authors:Zhang Fan  Zhuang Huisheng  Wang Qiong'e
Institution:Department of Chemistry, Fuzhou Univeraity, Fuzhou 350002
Abstract:A new chemiluminescence analytical system of xanthine oxidase-acetaldehyde-Acridinium was studied.This system is based on the quantitative oxidation of acetaldehyde by xanthine oxidase to form acetic acid and hydrogen peroxide.The hydrogen peroxide produced is then measured by a new acridinium ester 10-methyl-9-(p-formylphenyl)acridinium carboxylate fluorosulphonate]CL system.In addition, the effect of other substances on the test was investigated.The appropriate conditions for the ezymatic reaction and the chemiluminescent reaction were studied.In addition, the effect of other substances on the acetaldehyde determination was also investigated.The graph was linear over three orders of magnitude between 2.6×10-7 and 1.2×10-3mol/L and the relative standard deviation (n=10) was 6.8% for a 5.0×10-7mol/L acetaldehyde.The detection limit for acetaldehyde by this method was about one order of magnitude better than those of other chemiluminescence methods; The determination of acetaldehyde in certain polluted water, the results were satisfactory.
Keywords:xanthine oxidase          acetaldehyde          chemiluminescence analysis          10-Methyl-9(p-formylphenyl)-acridinium carbxylate fluorosulphonate
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