| 基于InP@ZnS QDs/Dured纳米荧光探针的DNA检测 |
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| 引用本文： | 胡先运,孟铁宏,张汝国,江家志,黄星宏.基于InP@ZnS QDs/Dured纳米荧光探针的DNA检测[J].发光学报,2017,38(3):288-295. |
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| 作者姓名： | 胡先运 孟铁宏 张汝国 江家志 黄星宏 |
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| 作者单位： | 1. 东南大学生物科学与医学工程学院 生物电子学国家重点实验室, 江苏 南京 210096;
2. 黔南民族医学高等专科学校, 贵州 都匀 558000 |
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| 基金项目： | 国家自然科学基金，贵州省自然科学基金，中央高校基本科研业务费专项资金，黔南民族医专科研基金(QNYZ201601)资助项目 Supported by National Natural Science Foundation of China，Natural Science Foundation of Guizhou Province，Special Fund for Basic Scientific Research of Central University，National Medical Research Foundation of Qiannan |
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| 摘 要： | 利用巯基丙酸包覆的In P@Zn S量子点(QDs)与Dured构建了一种检测DNA的荧光探针。在该探针中,以环境友好型带负电的In P@Zn S量子点为荧光团,与带正电的Dured通过静电结合,构建了In P@Zn S QDs/Dured纳米荧光探针。通过荧光共振能量转移(FRET)机理,量子点荧光被猝灭;当DNA存在时,Dured与DNA的特异性结合使Dured从In P@Zn S QDs表面脱附,FRET过程被打断,In P@Zn S QDs荧光恢复,以荧光"关-开"方式检测DNA。该探针检测DNA的线性范围为2.0~275.0 ng·L-1,检测限为1.0 ng·L-1,并可用于模拟生物生理条件下的DNA检测。
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| 关 键 词： | InP@ZnS量子点 荧光探针 DNA 荧光共振能量转移 |
| 收稿时间： | 2016-09-19 |
InP@ZnS QDs/Dured Fluorescent Nanoprobe for The Detection of DNA |
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| HU Xian-yun,MENG Tie-hong,ZHANG Ru-guo,JIANG Jia-zhi,HUANG Xing-hong.InP@ZnS QDs/Dured Fluorescent Nanoprobe for The Detection of DNA[J].Chinese Journal of Luminescence,2017,38(3):288-295. |
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| Authors: | HU Xian-yun MENG Tie-hong ZHANG Ru-guo JIANG Jia-zhi HUANG Xing-hong |
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| Institution: | 1. State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China;
2. Qiannan Medical College for Nationalities, Duyun 558000, China |
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| Abstract: | The fluorescent nanoprobe for the detection of DNA was established by utilizing mercaptopropionic acid coated InP@ZnS QDs and Dured.In this nanoprobe.The InP@ZnS QDs/Dured fluorescent nanoprobes were structured by electrostatic interactions between environment friendly,negatively charged InP@ZnS quantum dots and positively charged Dured,and then the fluorescence of InP@ZnS QDs/Dured was quenched through fluorescence resonance energy transfer (FRET).At presence of DNA,DNA and Dured were specific binding,which enable Dured removing from the surface of InP@ZnS QDs and achieving fluorescence recovery of InP@ZnS QDs.Thus,the detection of DNA was realized.The linear range of the InP@ZnS QDs/Dured fluorescent nanoprobes for DNA detection and the detection limit were 2.0-275.0 ng·L-1 and 1.0 ng·L-1,respectively.The InP@ZnS QDs/Dured fluorescent nanoprobes can be also used in rapid detection of DNA under simulated physiological conditions. |
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| Keywords: | InP@ZnS quantum dots fluorescent probe DNA FRET |
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