| 丁二酰化壳寡糖铕配合物的合成及与牛血清白蛋白的相互作用 |
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| 引用本文： | 李小芳,冯小强,杨声,张玉霞.丁二酰化壳寡糖铕配合物的合成及与牛血清白蛋白的相互作用[J].发光学报,2012,33(8):905-910. |
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| 作者姓名： | 李小芳 冯小强 杨声 张玉霞 |
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| 作者单位： | 1. 天水师范学院 生命科学与化学学院, 甘肃 天水 741001;2. 定西师范高等专科学校, 甘肃 定西 743000 |
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| 基金项目： | 天水师范学院物理无机化学重点学科基金(ZD0840),天水师范学院“青蓝”人才工程,天水师范学院中青年教师科研项目(TSA1003)资助项目 |
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| 摘 要： | 合成并表征了丁二酰化壳寡糖铕配合物,在模拟人体生理条件下,运用紫外和荧光光谱研究了配合物与牛血清白蛋白(BSA)的相互作用.结果表明:随着配合物浓度的增加,BSA的紫外光谱表现出增色效应并伴有蓝移;配合物与BSA形成复合物,从而猝灭BSA内源荧光,该猝灭机制为静态猝灭.计算得到室温下配合物与BSA的结合常数和结合位点数...
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| 关 键 词： | 丁二酰化壳寡糖铕 牛血清白蛋白 相互作用 |
| 收稿时间： | 2012/5/7 |
Synthesis and Interaction of Succinic-oligochitosan-Eu(Ⅲ) Complex with Bovine Serum Albumin |
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| LI Xiao-fang
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FENG Xiao-qiang
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YANG Sheng
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ZHANG Yu-xia.Synthesis and Interaction of Succinic-oligochitosan-Eu(Ⅲ) Complex with Bovine Serum Albumin[J].Chinese Journal of Luminescence,2012,33(8):905-910. |
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| Authors: | LI Xiao-fang FENG Xiao-qiang YANG Sheng ZHANG Yu-xia |
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| Institution: | 1. College of Life Science and Chemistry, Tianshui Normal University, Tianshui 741001, China;2. Dingxi Teachers College, Dingxi 743000, China |
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| Abstract: | Succinic-oligochitosan-Eu(Ⅲ) complex was synthesized and characterized.Under simulated physiological conditions,the interaction between complex and bovine serum albumins(BSA) was studied by ultraviolet spectrum and fluorescence spectrum.The results showed that the absorption intensity of BSA increased with the concentration of complex.The complex quenched the fluorescence of BSA and it was static quenching,and then the binding constant and binding sites were calculated.They were 2.068 2×104 L·mol-1 and 1.094 72 at room temperature,respectively. |
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| Keywords: | succinic-oligochitosan-Eu(Ⅲ) bovine serum albumin interaction |
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