| 稀土La(Ⅲ)、Nd(Ⅲ)与牛血清白蛋白的相互作用 |
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| 引用本文： | 冯小强,伏国庆,李小芳,杨声,苏中兴.稀土La(Ⅲ)、Nd(Ⅲ)与牛血清白蛋白的相互作用[J].发光学报,2011,32(2):205-209. |
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| 作者姓名： | 冯小强 伏国庆 李小芳 杨声 苏中兴 |
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| 作者单位： | 1. 天水师范学院 生命科学与化学学院, 甘肃 天水 741001;
2. 天水市中医医院, 甘肃 天水 741001;
3. 兰州大学 化学化工学院, 甘肃 兰州 730030 |
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| 基金项目： | 甘肃天水师范学院物理无机化学重点学科基金 |
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| 摘 要： | 采用紫外吸收、荧光光谱和红外光谱,研究了稀土离子La(Ⅲ)和Nd(Ⅲ)与牛血清白蛋白(BSA)的相互作用.结果表明:La(Ⅲ)和Nd(Ⅲ)离子能配位诱导BSA,使其分子中色氨酸和酪氨酸芳杂环疏水基团外露,在280 nm处的吸收增强而表现出增色效应和较小的蓝移.La(Ⅲ)和Nd(Ⅲ)可以有规律地猝灭BSA的内源荧光,其猝...
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| 关 键 词： | 稀土 牛血清白蛋白 紫外光谱 红外光谱 荧光光谱 |
| 收稿时间： | 2010-06-04 |
Interaction of Rare Earth with Bovine Serum Albumin |
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| FENG Xiao-qiang,FU Guo-qing,LI Xiao-fang,YANG Sheng,SU Zhong-xing.Interaction of Rare Earth with Bovine Serum Albumin[J].Chinese Journal of Luminescence,2011,32(2):205-209. |
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| Authors: | FENG Xiao-qiang FU Guo-qing LI Xiao-fang YANG Sheng SU Zhong-xing |
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| Institution: | 1. College of Life Science and Chemistry Tianshui Normal University, Tianshui 741001, China;
2. Tianshui Traditional Chinese Medical Hospital, Tianshui 741001, China;
3. Chemistry and Chemical Engineering College, Lanzhou University, Lanzhou 730000, China |
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| Abstract: | The interaction of rare earth La (Ⅲ) and Nd (Ⅲ) with BSA were studied by ultraviolet spectrum,fluorescence spectrum and IR spectrum. The results showed that the ultraviolet absorption intensity of BSA increases with the concentration of rare earth. Rare earth can strongly quench the fluorescence of BSA which is the static quenching. The binding constant (KA) of La (Ⅲ) or Nd (Ⅲ) with BSA at room temperature are 8.12×106 and 3.85×106, respectively. |
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| Keywords: | rare earth bovine serum albumin ultraviolet spectrum IR spectrum fluorescence spectrum |
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