Role of non-lamellar-forming lipid in promotion of liposomal fusion |
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Authors: | Suman Bhandary Ruma Basu Smarajit Manna Sukhen Das Papiya Nandy |
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Institution: | 1. Department of Physics , Jadavpur University , Raja S.C. Mallick Road, Kolkata 700032, India suman_bhandary@yahoo.co.in;3. Department of Physics , Jogamaya Devi College , Kolkata 700026, India;4. Department of Physics , Jadavpur University , Raja S.C. Mallick Road, Kolkata 700032, India |
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Abstract: | Membrane fusion is an important process in a wide range of cellular and sub-cellular activities. It is evident that during the intermediate stages of fusion some transitory non-bilayer configurations must appear within the lipid moiety. Using fluorescence techniques, we have studied here the process of aggregation and fusion of liposomes made of lipids, namely 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC). When mixed together, the complete fusion between these two liposomes took around 44 h as both DPPC and DMPC favour lamellar configuration. When the mixture was incubated at 42°C the fusion process was completed after 23 h. But, when 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) was added in the liposomal matrix the time for fusion was reduced to 21 h for mixture without incubation and 17 h when the mixture was incubated. This indicates that DPPE having a tendency to assume non-lamellar conformation, promoted destabilisation of the lamellar conformation within the liposome which facilitated the fusion between two apposing bilayers. |
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Keywords: | membrane fusion lipid polymorphism fluorescence anisotropy phase transition temperature |
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