A high-sensitivity photonic crystal fiber (PCF) based on the surface plasmon resonance (SPR) biosensor for detection of density alteration in non-physiological cells (DANCE) |
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| Authors: | F Wang Z Sun C Liu T Sun PK Chu |
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| Institution: | 1. Institute of Materials Processing and Intelligent Manufacturing & Center for Biomedical Materials and Engineering, Harbin Engineering University, Harbin 150001, China;2. School of Electronics Science, Northeast Petroleum University, Daqing 163318, China;3. Institute of Microelectronics, Agency of Science, Technology and Research (A*STAR), 117685, Singapore;4. Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China |
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| Abstract: | A highly sensitive photonic crystal fiber based on the surface plasmon resonance (PCF-SPR) biosensor for the detection of the density alteration in non-physiological cells (DANCE) is described. Human acute leukemia cells are determined by the discontinuous sucrose gradient centrifugation (DSGC) in which the cells are separated into several bands. The separated cells with different intracellular densities and refractive indexes (RI) ranging from 1.3342 to 1.3344 are distinguished in situ by means of the differential transmission spectrum. The biosensor shows a maximum amplitude sensitivity of 2000?nm/RIU and resolution as high as 5?×?10?5?RIU. According to the wavelength interrogation method, a maximum spectral sensitivity of 9000?nm/RIU in the sensing range between 1.33 and 1.53 is achieved, corresponding to a resolution as high as 1.11?×?10?5?RIU for the biosensor. The proposed PCF-SPR biosensor has promising application in biological and biochemical detection. |
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| Keywords: | PCF-SPR biosensor DNACE Sensitivity Resolution |
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