Speckle variance detection of microvasculature using swept-source optical coherence tomography |
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Authors: | Mariampillai Adrian Standish Beau A Moriyama Eduardo H Khurana Mamta Munce Nigel R Leung Michael K Jiang James Cable Alex Wilson Brian C Vitkin I Alex Yang Victor X D |
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Institution: | Department of Medical Biophysics, University of Toronto, Toronto, M5G 2M9, Canada. |
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Abstract: | We report on imaging of microcirculation by calculating the speckle variance of optical coherence tomography (OCT) structural images acquired using a Fourier domain mode-locked swept-wavelength laser. The algorithm calculates interframe speckle variance in two-dimensional and three-dimensional OCT data sets and shows little dependence to the Doppler angle ranging from 75 degrees to 90 degrees . We demonstrate in vivo detection of blood flow in vessels as small as 25 microm in diameter in a dorsal skinfold window chamber model with direct comparison with intravital fluorescence confocal microscopy. This technique can visualize vessel-size-dependent vascular shutdown and transient vascular occlusion during Visudyne photodynamic therapy and may provide opportunities for studying therapeutic effects of antivascular treatments without on exogenous contrast agent. |
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