In vivo video rate multiphoton microscopy imaging of human skin |
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Authors: | Lee Anthony M D Wang Hequn Yu Yingqiu Tang Shuo Zhao Jianhua Lui Harvey McLean David I Zeng Haishan |
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Institution: | Integrative Oncology Department-Imaging Unit, British Columbia Cancer Agency Research Centre, Vancouver, British Columbia V5Z 1L3, Canada. |
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Abstract: | We present a multiphoton microscopy instrument specially designed for in vivo dermatological use that is capable of imaging human skin at 27 frames per second with 256 pixels × 256 pixels resolution without the use of exogenous contrast agents. Imaging at fast frame rates is critical to reducing image blurring due to patient motion and to providing practically short clinical measurement times. Second harmonic generation and two-photon fluorescence images and videos acquired at optimized wavelengths are presented showing cellular and tissue structures from the skin surface down to the reticular dermis. |
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