Spectrofluorimetric determination of nucleic acids as 8-hydroxyquinoline/ yttrium ternary complexes |
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Authors: | Cheng Zhi Huang Ke An Li Shen Yang Tong |
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Institution: | (1) Institute of Analytical Chemistry, Faculty of Chemistry and Molecular Engineering, Peking University, 100871 Beijing, Peoples Republic of China;(2) Present address: Institute of Environmental Chemistry, Southwest Normal University, 630715 Chongqing, Peoples Republic of China |
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Abstract: | The formation of nucleic acids/8-hydroxyquinoline/yttrium(III) ternary complexes and their fluorescent properties have been studied. The nucleic acids studied include native and thermally denatured calf thymus DNA, fish sperm DNA and yeast RNA. In the range of pH 7.6–8.5, controlled by NH3-NH4C1 buffer, ternary complexes are formed that fluoresce at different wavelengths with different nucleic acids. Based on the fluorescence reactions, sensitive spectrofluorimetric methods for nucleic acids are proposed. In optimal conditions, the calibration curves were linear in the range 0.5–4.0 gml–1 for calf thymus DNA, 0.5–2.5 g ml–1 for fish sperm DNA and 0.5–4.0 g ml–1 for yeast RNA. The limits of determination (3 ) were 0.030 g ml–1 for calf thymus DNA, 0.020 g ml–1 for fish sperm DNA and 0.090 g ml–1 for yeast RNA. Corresponding to the interferences of coexisting substances, six synthetic samples were constructed and the results of determination were satisfactory. |
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Keywords: | spectrofluorimetry nucleic acids DNA RNA 8-hydroxyquinoline yttrium |
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