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| Solid-phase Synthesis of Acyl-plasminogen-Streptokinase Activator Composite（APSAC） and Its Thrombolytic Properties | |
| 引用本文： | TENGLi-rong LIANGFang SUNHong QINYi HONGShui-sheng CHENJia LIULan-ying. Solid-phase Synthesis of Acyl-plasminogen-Streptokinase Activator Composite（APSAC） and Its Thrombolytic Properties[J]. 高等学校化学研究, 2004, 20(5): 584-587 |
| 作者姓名： | TENGLi-rong LIANGFang SUNHong QINYi HONGShui-sheng CHENJia LIULan-ying |
| 作者单位： | [1]CollegeofLifeScience,JilinUniversity,Changchun130023,P.R.China [2]InstituteofBiologicalProducts,Changchun130062,P.R.China [3]DepartmentofPharmacology,ShenyangPharmaceuticalUniversity,Shenyang110016,P.R.China |
| 基金项目： | 吉林省自然科学基金，the Creative Foundation of Jilin University |
| 摘 要： | The dialysis method has been traditionally used for the conversion of native human plasminogen (Glu-Hpg) to lys-plasminogen(Lys-Hpg). Here is described a solid-phase synthesis method for the preparation of an acyl-plasminogen-streptokinase activator complex(APSAC) from Lys-Hpg, streptokinase (SK) and chemical modification agent(4-amidinophenyl-4‘-aminobenzoate hydrochloride) with the L-lysine-Sepharose 4B Column as the carrier. The new method significantly increases the product yield and purity over the liquidphase methods. The APSAC prepared with the new method exhibits a significant thrombolytic effect with a long half-life of about 8.8 h in rabbits. |
| 关 键 词： | 固相合成 溶血栓特性 酰基血浆酶原溶栓酶活化剂 赖氨酸 谷氨酸 |
Solid-phase Synthesis of Acyl-plasminogen-Streptokinase Activator Composite(APSAC) and Its Thrombolytic Properties |
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| TENG Li-rong,LIANG Fang,SUN Hong,QIN Yi,HONG Shui-sheng,CHEN Jia. Solid-phase Synthesis of Acyl-plasminogen-Streptokinase Activator Composite(APSAC) and Its Thrombolytic Properties[J]. Chemical Research in Chinese University, 2004, 20(5): 584-587 | |
| Authors: | TENG Li-rong LIANG Fang SUN Hong QIN Yi HONG Shui-sheng CHEN Jia |
| Abstract: | The dialysis method has been traditionally used for the conversion of native human plasminogen (GluHpg) to lys-plasminogen(Lys-Hpg). Here is described a solid-phase synthesis method for the preparation of an acyl-plasminogen-streptokinase activator complex(APSAC) from Lys-Hpg, streptokinase (SK) and chemical modification agent(4-amidinophenyl-4'-aminobenzoate hydrochloride) with the L-lysine-Sepharose 4B Column as the carrier. The new method significantly increases the product yield and purity over the liquidphase methods. The APSAC prepared with the new method exhibits a significant thrombolytic effect with a long half-life of about 8. 8 h in rabbits. |
| Keywords: | Acyl-plasminogen-streptokinase activator complex (APSAC) Solid-phase synthesis Thrombolytic |
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