High resolution liquid chromatography with a packed micro glass capillary column

Single, long columns (1–5 m) can be prepared efficiently using reversed phase packings (3–10 μm particle diameter). 1-m columns packed with 3 and 10 μm packing provide 110 000 and 50 000 theoretical plates, respectively. Very efficient columns can resolve highly complex mixtures and difficult-to-separate compounds. Temperature gradient elution is a powerful technique for LC with microbore columns.