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Gas chromatographic separation of sugars by on-column injection on glass capillary columns |
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| Authors: | H Traitler S Del Vedovo T F Schweizer |
| Abstract: | Sugars were separated gas chromatographically on short apolar glass capillary columns by using cold, on-column injection (OCI) techniques. After silylation, oligomers up to the hexasaccharides could be efficiently separated in resonable retention times. Response factors of silylated sugars were determined as a function of varying sample amounts and concentrations. The optimum injection amount was found to be 1 μl in heptane as solvent. |
| Keywords: | Gas chromatography GC Capillary columns glass On-column injection Sugars Oligosaccharides |