Development of a fast capillary electrophoresis method for the determination of propranolol—Total analysis time reduction strategies |
| |
Authors: | Gustavo Amadeu Micke Ana Carolina O Costa Melina Heller Michelle Barcellos Marcel Piovezan Thiago Caon Marcone Augusto Leal de Oliveira |
| |
Institution: | 1. Department of Chemistry, Federal University of Santa Catarina, 88040-900 Florianopolis, SC, Brazil;2. Department of Chemistry, Federal University of Juiz de Fora, 36036-330 Juiz de Fora, MG, Brazil;3. Institute of Chemistry, University of Sao Paulo, 05508-000 Sao Paulo, SP, Brazil |
| |
Abstract: | The aim of this study was to develop a fast capillary electrophoresis method for the determination of propranolol in pharmaceutical preparations. In the method development the pH and constituents of the background electrolyte were selected using the effective mobility versus pH curves. Benzylamine was used as the internal standard. The background electrolyte was composed of 60 mmol L−1 tris(hydroxymethyl)aminomethane and 30 mmol L−1 2-hydroxyisobutyric acid, at pH 8.1. Separation was conducted in a fused-silica capillary (32 cm total length and 8.5 cm effective length, 50 μm I.D.) with a short-end injection configuration and direct UV detection at 214 nm. The run time was only 14 s. Three different strategies were studied in order to develop a fast CE method with low total analysis time for propranolol analysis: low flush time (Lflush) 35 runs/h, without flush (Wflush) 52 runs/h, and Invert (switched polarity) 45 runs/h. Since the three strategies developed are statistically equivalent, Wflush was selected due to the higher analytical frequency in comparison with the other methods. A few figures of merit of the proposed method include: good linearity (R2 > 0.9999); limit of detection of 0.5 mg L−1; inter-day precision better than 1.03% (n = 9) and recovery in the range of 95.1–104.5%. |
| |
Keywords: | Propranolol Total analysis time Short-end injection Fast method Effective mobility curves Peakmaster |
本文献已被 ScienceDirect 等数据库收录! |
|